# J1053: DEFERRISOMA PALEOCHRORII MEDIUM


## Main sol. J1053

1. Mix components thoroughly, adjust pH to 6.0 with 1 N HCl and autoclave under a H2-CO2 (4:1, v/v) gas mixture. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): 

15	g	NaCl	
4	g	MgCl2 x 6 H2O	
0.33	g	KCl	
0.33	g	CaCl2 x 2 H2O	
0.5	g	(NH4)2SO4	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
0.5	ml	**Selenite-tungstate solution**	
860	ml	Distilled water	
10	ml	KH2PO4 (3.3%)	
10	ml	**Trace vitamins** (*)	
10	ml	NaHCO3 (*, 8%)	
10	ml	 (w/v) MES (pH 6.0, 20%)	
100	ml	**Iron(III) citrate solution**	

2. Readjust pH to 6.0 to 6.3, if necessary.


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Selenite-tungstate solution

0.4	g	NaOH	
6	mg	Na2SeO3 x 5 H2O	
8	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	


## Iron(III) citrate solution

1. Mix 2.4 g iron(III) citrate and 90 ml distilled water, and boil until dissolved. Cool to an ambient temperature and adjust pH to 6.0 with 10 N NaOH solution. Fill up to 100 ml with distilled water.