# J1063: SEA SALTS YEAST EXTRACT GLUCOSE MEDIUM


## Main sol. J1063

30	g	Sea Salt (Sigma)	
0.15	g	KH2PO4	
0.15	g	K2HPO4	
2	g	NH4Cl	
2	g	Yeast extract	
5	g	Glucose	
0.5	g	L-Cysteine HCl x H2O	
1	g	NaHCO3	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Mix components except NaHCO3 and adjust pH to 7.0. Bring to a boil, cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream and add NaHCO3. Dispense the medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave.