# J1064: GEOSPOROBACTER MEDIUM ## Main sol. J1064 0.5 g KCl 0.2 g KH2PO4 1 g NaCl 0.05 g NH4Cl 0.1 g CaCl2 x 2 H2O 0.4 g MgCl2 x 6 H2O 1 g Yeast extract 0.36 g Glucose 1 ml **Trace vitamins** 1 ml **Trace element solution** 0.84 g NaHCO3 0.5 g Na2S x 9 H2O 1 mg Resazurin 1000 ml Distilled water 1. Mix ingredients except NaHCO3, Trace vitamins and Na2S x 9H2O, and autoclave under a N2-CO2 (90:10) atmosphere. Filter-sterilize NaHCO3 as an 8.4% solution and Trace vitamins while gassing with N2. Separately autoclave Na2S x 9H2O as 5% solution under a N2 atmosphere. Aseptically and anaerobically add NaHCO3 and Trace vitamins to the medium under a N2-CO2 (90:10) atmosphere. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 7.5 - 8.0. ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water ## Trace element solution 0.64 g Na2-EDTA 6.2 g MgSO4 x 7 H2O 0.55 g MnSO4 x n H2O 1 g NaCl 0.1 g FeSO4 x 7 H2O 0.17 g CoCl2 x 6 H2O 0.13 g CaCl2 x 2 H2O 0.18 g ZnSO4 x 7 H2O 0.05 g CuSO4 x 5 H2O 0.018 g AlK(SO4)2 x 12 H2O 0.01 g H3BO3 0.011 g Na2MoO4 x 2 H2O 0.025 g NiCl2 x 6 H2O 1. Dissolve EDTA x 2Na first in 500 ml of distilled water. Then add the remaining salts and adjust volume to 1000 ml.