# J1066: M25Y MEDIUM


## Main sol. J1066

3.9	g	MES	
25	g	NaCl	
2.7	g	MgSO4 x 7 H2O	
4.3	g	MgCl2 x 6 H2O	
0.25	g	NH4Cl	
0.34	g	KCl	
0.14	g	CaCl2 x 2 H2O	
0.14	g	K2HPO4	
0.2	g	Yeast extract	
1	ml	**Trace elements**	
1	ml	Fe(NH4)2(SO4)2 x 6 H2O (in 0.037% HCl, 0.2%)	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Adjust pH to 5.5. Autoclave under a N2 atmosphere. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter sterilized): 

10	ml	Trace vitamins (see Medium No. 197) (*)	
10	ml	Na2S x 9 H2O (5%)	


## Trace elements

29	g	Na2-EDTA	
3.26	g	MnSO4 x n H2O	
1.8	g	CoCl2 x 6 H2O	
1	g	ZnSO4 x 7 H2O	
0.11	g	NiSO4 x 6 H2O	
0.1	g	CuSO4 x 5 H2O	
0.1	g	H3BO3	
0.1	g	AlK(SO4)2 x 12 H2O	
0.1	g	Na2MoO4 x 2 H2O	
0.1	g	Na2WO4 x 2 H2O	
0.05	g	Na2SeO3	
1000	ml	Distilled water	

1. First dissolve EDTA x 2Na and adjust pH to 8.0 with KOH. Then add the minerals.