# J1073: MARINE SRB MEDIUM


## Main sol. J1073

3	g	Na2SO4	
0.2	g	KH2PO4	
0.3	g	NH4Cl	
13.5	g	NaCl	
2.2	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.15	g	CaCl2 x 2 H2O	
3	μg	Na2SeO3	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
10	ml	**Trace vitamins**	
0.5	g	Yeast extract	
1	mg	Resazurin	
950	ml	Distilled water	

1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized): 

30	ml	NaHCO3 (*, 8%)	
20	ml	L-Sodium lactate (10%)	

2. Aseptically distribute the medium into culture vessels under and a N2-CO2 (4:1, v/v) gas stream and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution (autoclaved and stored under a N2 atmosphere): 

6	ml	Na2S x 9 H2O (5%)	


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water