# J1088: 0.3 x HYPHOMICROBIUM MEDIUM


## Main sol. J1088

0.85	g	K2HPO4	
0.75	g	NaH2PO4	
0.2	g	(NH4)2SO4	
0.07	g	MgSO4 x 7 H2O	
1	ml	**Visniac trace elements**	
2	ml	**Trace vitamins**	
2	ml	Methanol	
15	g	Agar	
1000	ml	Distilled water	

1. After autoclaving, aseptically add filter-sterilized trace vitamins solution and methanol.


## Trace vitamins

125	mg	Vitamin B12	
25	mg	Thiamine HCl	
12.5	mg	Folic acid	
40	mg	Ascorbic acid	
25	mg	Riboflavin	
25	mg	Niacin	
25	mg	Pantothenic acid	
500	ml	Distilled water	


## Visniac trace elements

5	g	Na2-EDTA	
2.2	g	ZnSO4 x 7 H2O	
0.733	g	CaCl2 x 2 H2O	
0.506	g	MnCl2 x 4 H2O	
0.499	g	FeSO4 x 7 H2O	
0.11	g	(NH4)6Mo7O24 x 4 H2O	
0.157	g	CuSO4 x 5 H2O	
0.161	g	CoCl2 x 6 H2O	
500	ml	Distilled water