# J1096: PYGA MEDIUM ## Main sol. J1096 ## Solution 1 8 g Peptone 3 g Yeast extract 1 g K2HPO4 10 g Glucose 15 g Agar 900 ml Distilled water ## Solution 2 2.9 g NaHCO3 7 g Na2CO3 100 ml Distilled water 1. Autoclave Solutions 1 and 2 separately and mix them aseptically.