# J1098: BSM-RC MEDIUM


## Main sol. J1098

3.62	g	NaCl	
0.26	g	K2HPO4	
0.075	g	NH4Cl	
0.05	g	Na2SO4	
0.85	g	NaNO3	
2.24	g	L-Sodium lactate	
0.2	g	Yeast extract	
0.5	g	Tryptone	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
10	ml	**Trace vitamins**	
935.5	ml	Distilled water	

1. Mix components thoroughly and autoclave under a N2 atmosphere. After cooling, add the following solution (filter-sterilized): 

52.5	ml	NaHCO3 (8%)	

2. Aseptically and anaerobically distribute the medium into culture vessels under a N2 atmosphere and add H2 at 5% volume of the gas phase.


## Trace element solution

3	g	MgCl2 x 6 H2O	
100	mg	CaCl2 x 2 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water