# J1106: MARINE METHANOGENIC MEDIUM ## Main sol. J1106 22 g NaCl 0.14 g CaCl2 x 2 H2O 0.34 g KCl 0.5 g NH4Cl 2.75 g MgCl2 x 6 H2O 0.14 g K2HPO4 0.2 g Yeast extract (BD-Difco) 10 ml **Trace mineral solution** 1 mg Resazurin 930 ml Distilled water 1. Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): 30 ml NaHCO3 (*, 8%) 10 ml **Trace vitamins** (*) 10 ml Na2S x 9 H2O (5%) 10 ml L-Cysteine HCl x H2O (5%) ## Trace mineral solution 1000 ml **Trace minerals** 0.03 g NiCl2 x 6 H2O 0.3 mg Na2SeO3 x 5 H2O 0.4 mg Na2WO4 x 2 H2O ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water