# J115: MODIFIED CM+YE MEDIUM (A) ## Main sol. J115 7.5 g Casamino acids (BD-Difco), vitamin assay 10 g Yeast extract (BD-Difco) 20 g MgSO4 x 7 H2O 3 g Trisodium citrate x 2 H2O 2 g KCl 150 g NaCl 1 ml **Fe2+ solution 49.8 g/L** 15 g Agar 1000 ml Distilled water 1. Adjust pH to 7.4. ## Fe2+ solution 49.8 g/L 4.98 g FeSO4 x 7 H2O 100 ml Distilled water