# J1151: DESULFOBACTERIUM NAPHTHALENIVOLANS MEDIUM


## Main sol. J1151

1	g	NaCl	
0.5	g	KCl	
0.4	g	MgCl2 x 6 H2O	
0.2	g	KH2PO4	
0.25	g	NH4Cl	
0.15	g	CaCl2 x 2 H2O	
1.42	g	Na2SO4	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
1	ml	**Selenite-tungstate solution**	
0.4	mg	Resazurin	
970	ml	Distilled water	

1. Mix components and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (filter-sterilized): 

1	ml	**Vitamin solution**	
30	ml	NaHCO3 (8%)	

2. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 50 ml of the medium in 120 ml serum bottle) under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Prior to use, add per liter the following solution (autoclaved and stored under a N2 atmosphere): 

20	ml	**Naphthalene solution** (1.5%)	
4	ml	Na2S x 9 H2O (5%)	


## Naphthalene solution

1.5	g	Naphthalene	
100	ml	2,2,4,4,6,8,8-Heptamethylnonane	


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Selenite-tungstate solution

0.4	g	NaOH	
6	mg	Na2SeO3 x 5 H2O	
8	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water	


## Vitamin solution

20	mg	Biotin	
200	mg	Nicotinic acid	
100	mg	p-Aminobenzoic acid	
200	mg	Thiamine HCl	
100	mg	DL-Calcium pantothenate	
500	mg	Pyridoxine hydrochloride	
100	mg	Vitamin B12	
100	mg	Riboflavin	
1000	ml	Distilled water	

1. Filter-sterilize and store under a N2 atmosphere.