# J1155: DESULFOOBULBUS OLIGOTROPHICUS MEDIUM


## Main sol. J1155

0.5	g	KH2PO4	
0.5	g	K2HPO4	
0.5	g	NH4Cl	
5	g	NaCl	
2.84	g	Na2SO4	
0.3	g	MgCl2 x 6 H2O	
0.05	g	CaCl2 x 2 H2O	
0.4	g	KCl	
1	ml	Trace element solution (see Medium No. 294)	
1	mg	Resazurin	
955	ml	Distilled water	

1. Mix components thoroughly, adjust pH to 7.6 with KOH and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions (autoclaved or *filter-sterilized): 

25	ml	NaHCO3 (*, 8%)	
20	ml	Sodium propionate (1 M)	
1	ml	**Trace vitamins** (*)	

2. Aseptically and anaerobically distribute the medium into culture vessels (e.g., 20 ml medium in 60 ml serum bottles) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add per liter the following solution (autoclaved and stored under N2): 

8	ml	Na2S x 9 H2O (5%)	

3. Readjust pH to 7.6, if necessary.


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water