# J1157: DESULFONATRONUM PAR180 MEDIUM


## Main sol. J1157

5	g	NaCl	
0.2	g	KH2PO4	
4	g	Na2SO4	
3.5	g	Na2CO3	
0.25	g	NH4Cl	
0.1	g	MgCl2 x 6 H2O	
0.2	g	KCl	
0.2	g	Yeast extract	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
1	ml	**Fe-Ni-WO-Se solution**	
1000	ml	Distilled water	

1. Mix components thoroughly and adjust pH to 9.0. Bring to a boil and cool under a N2 gas stream. Distribute the medium into culture vessels (e.g., 10 ml of the medium in the Hungate tubes) under a N2 atmosphere, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions (autoclaved or *filter-sterilized and stored under a N2 atmosphere): 

1	ml	Vitamin solution (see below, *)	
20	ml	Sodium lactate (1 M)	
8	ml	Na2S x 9 H2O (5%)	

2. Vitamin solution: 

10	mg	Thiamine HCl	
20	mg	Nicotinic acid	
5	mg	DL-Calcium pantothenate	
50	mg	Pyridoxine hydrochloride	
2	mg	Biotin	
10	mg	p-Aminobenzoic acid	
20	mg	Vitamin B12	
1000	ml	Distilled water	


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Fe-Ni-WO-Se solution

1.42	g	FeSO4 x 7 H2O	
1.6	g	NiSO4 x 7 H2O	
38	mg	Na2WO4 x 2 H2O	
3	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water