# J1180: CELLULOLYTIC NATRONOARCHAEA MEDIUM


## Main sol. J1180

128	g	NaCl	
95	g	Na2CO3	
15	g	NaHCO3	
2.5	g	KCl	
1.75	g	K2HPO4	

1. Add components to distilled water and bring volume to 1.0 L. After cooling, add the following solutions (autoclaved or *filter-sterilized): 

1.25	ml	NH4Cl (10%)	
1	ml	MgSO4 (1 M)	
1	ml	**Trace element solution**	
5	ml	**Trace vitamins** (*)	
0.2	ml	Yeast extract (10%)	
20	ml	Cellobiose (10%)	


## Trace element solution

5	g	EDTA	
2	g	FeSO4 x 7 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuCl2 x 2 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Adjust pH to 3.6.


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water