# J1228: ANAEROBIC ALKALINE GLUCOSE MEDIUM


## Main sol. J1228

20	g	NaCl	
50	g	Na2CO3	
60	g	NaHCO3	
900	ml	Distilled water	

1. Solution B: 

0.1	g	MgCl2 x 6 H2O	
0.5	g	NH4Cl	
0.2	g	KH2PO4	
0.05	g	Yeast extract	
3	g	Glucose	
1	ml	**Trace element solution**	
100	ml	Distilled water	

2. Aseptically and anaerobically combine Solutions A and B. Check final pH of the medium to be around 9.2. Add the following solution into the medium (filter-sterilized and stored under a N2 atmosphere): 

10	ml	**Trace vitamins**	

3. Comment: For JCM 32929, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): 

10	ml	Peptone (10%)	
6	ml	Na2S x 9 H2O (5%)	

4. Solution A:
5. Mix components thoroughly and autoclave under a N2 gas atmosphere.
6. Mix components thoroughly and autoclave under a N2 gas atmosphere.


## Trace element solution

784	mg	Fe(NH4)2(SO4)2 x 6 H2O	
238	mg	CoCl2 x 6 H2O	
395	mg	(NH4)2Ni(SO4)2 x 6 H2O	
24	mg	Na2MoO4 x 2 H2O	
33	mg	Na2WO4 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
2	mg	CuCl2 x 2 H2O	
94	mg	Na2SeO4	
6	mg	H3BO3	
99	mg	MnCl2 x 4 H2O	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water