# J1247: R2A MEDIUM WITH 25% SALT


## Main sol. J1247

0.5	g	Yeast extract (BD-Difco)	
0.5	g	Proteose peptone no. 3 (BD-Difco)	
0.5	g	Casamino acids (BD-Difco)	
0.5	g	Glucose	
0.5	g	Starch (soluble)	
0.3	g	Sodium pyruvate	
0.3	g	K2HPO4	
0.05	g	MgSO4 x 7 H2O	
833	ml	Sea water (see below, 30%)	
167	ml	Distilled water	

1. 30% <I>Seawater solution:</I> 

234	g	NaCl	
39	g	MgCl2 x 6 H2O	
61	g	MgSO4 x 7 H2O	
6	g	KCl	
1.3	g	CaCl2 x 2 H2O	
0.7	g	NaBr	
0.2	g	NaHCO3	

2. Adjust pH to 7.5.
3. Add components to distilled water and bring volume to 1.0 L.