# J1249: TYG-ACETATE MEDIUM


## Main sol. J1249

10	g	Tryptone	
10	g	Beef extract	
5	g	Yeast extract	
150	g	Malt extract	
20	g	Glucose	
2	g	Diammonium hydrogen citrate	
5	g	Sodium acetate	
2	g	K2HPO4	
0.19	g	MnSO4 x n H2O	
0.58	g	MgSO4 x 7 H2O	
1	ml	Tween 80	
1	g	L-Cysteine HCl x H2O	
1000	ml	Distilled water	

1. After autoclaving, aseptically add 20 ml acetic acid (filter-sterilized).