# J1273: 0.01% LB SEAWATER MEDIUM


## Main sol. J1273

120	mg	NaNO3	
5	mg	K2HPO4	
1	μg	Vitamin B12	
1	μg	Biotin	
100	μg	Thiamine HCl	
259	μg	Fe(III)-EDTA	
332	μg	Mn(II) x EDTA	
1	g	Tris(hydroxymethyl)aminomethane	
0.1	g	LB Broth (BD-Difco),  Lennox	
1000	ml	Sea water	

1. Mix components thoroughly, adjust pH to 8.0 with HCl and autoclave.
2. Comment: Artificial sea water (such as artificial sea water in Medium No. 1118) can be used instead of natural sea water.