# J1277: VULCANO HIGH SALT MEDIUM


## Main sol. J1277

18	g	NaCl	
1	g	MgCl2 x 2 H2O	
1	g	MgSO4 x 7 H2O	
0.3	g	KCl	
1	g	Na2SO4	
0.3	g	(NH4)2SO4	
0.05	g	K2HPO4	
0.05	g	KH2PO4	
0.02	g	CaCl2 x 2 H2O	
0.1	g	FeCl2 x 4 H2O	
0.1	g	Iron(III) citrate	
0.5	g	L-Cysteine HCl x H2O	
1.95	g	MES	
1	mg	Resazurin	
1	ml	**Trace element solution**	
1	ml	**Se-Ni-W solution**	
1000	ml	Distilled water	

1. Mix components thoroughly, adjust pH to 6.3 and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, add the following solutions from anaerobic stocks (autoclaved): 

5	ml	Yeast extract (10%)	
10	ml	Sodium fumarate (1.0 M)	

2. Comment:
3. For JCM 39183, add 1.0 M glucose solution (final 10 mM), instead of sodium fumarate solution, and adjust pH to 5.5.


## Trace element solution

12.5	ml	HCl (25%)	
2.1	g	FeSO4 x 7 H2O	
30	mg	H3BO3	
100	mg	MnCl2 x 4 H2O	
190	mg	CoCl2 x 6 H2O	
24	mg	NiCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
36	mg	Na2MoO4 x 2 H2O	
987	ml	Distilled water	


## Se-Ni-W solution

10	mg	Na2SeO3	
20	mg	NiCl2 x 6 H2O	
30	mg	Na2WO4 x 2 H2O	
100	ml	Distilled water