# J1283: SIUC-3 MEDIUM


## Main sol. J1283

3.6	g	HEPES	
3.24	g	Na2SO4	
0.17	g	K2HPO4	
0.4	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.3	g	NH4Cl	
0.25	g	NaHCO3	
6	mg	H3BO3	
1	ml	**Wolfe's mineral solution**	
0.68	g	Sodium formate	
0.1	g	Yeast extract	
0.2	mg	Resazurin	
1000	ml	Distilled water	

1. Mix components thoroughly and adjust pH to 6.5. Distribute the medium into culture vessels (e.g., 25 ml in 120 ml-serum bottles) under a stream of a N2 gas, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add per liter the following solution (autoclaved) : 

10	ml	Na2S x 9 H2O (5%)	


## Wolfe's mineral solution

1000	ml	**Trace minerals**	
0.02	g	NiCl2 x 6 H2O	
1	mg	Na2SeO3	
0.01	g	Na2WO4 x 2 H2O	


## Trace minerals

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x n H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.1	g	CoSO4 x 7 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.01	g	AlK(SO4)2	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.