# J1326: MODIFIED DESULFOVIBRIO MEDIUM ## Main sol. J1326 0.5 g K2HPO4 0.5 g KH2PO4 1 g NH4Cl 1 g Na2SO4 2 g MgSO4 x 7 H2O 0.1 g CaCl2 x 2 H2O 2 g Sodium lactate 1 g Yeast extract 1 ml **FeCl2 solution** 1 ml **Trace element solution** 10 ml **Trace vitamins** 1 mg Resazurin 980 ml Distilled water 1. SolutionB: 0.1 g FeSO4 x 7 H2O 10 ml Distilled water 2. SolutionC: 0.1 g Sodium thioglycolate 0.1 g Ascorbic acid 10 ml Distilled water 3. Solution A: 4. Dissolve ingredients of each solution in the appropriate amount of distilled water. Bring solution A to a boil and cool down to room temperature under a N2 gas stream. Add solutions Band C to the medium, then, adjust pH to 7.3 with NaOH. While swirling the medium, dispense into culture vessels under a N2 gas stream, seal with butyl rubber stoppers and autoclave. ## FeCl2 solution 10 ml HCl (7.7 M, 25%) 1.5 g FeCl2 x 4 H2O 990 ml Distilled water ## Trace element solution 70 mg ZnCl2 100 mg MnCl2 x 4 H2O 6 mg H3BO3 190 mg CoCl2 x 6 H2O 2 mg CuCl2 x 2 H2O 24 mg NiCl2 x 6 H2O 36 mg Na2MoO4 x 2 H2O 1000 ml Distilled water ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water