# J1328: PROLIXIBACTER MEDIUM


## Main sol. J1328

18	g	NaCl	
2.6	g	MgCl2 x 6 H2O	
0.15	g	CaCl2 x 2 H2O	
0.25	g	NH4Cl	
0.2	g	KH2PO4	
0.5	g	KCl	
0.85	g	NaNO3	
1.8	g	Glucose	
1	g	Yeast extract (BD-Difco)	
1	ml	Trace minerals (see Medium No. 243)	
1	ml	**Trace vitamins**	
2.52	g	NaHCO3	
1000	ml	Distilled water	

1. Mix components, and adjust pH to 7.0. Dispense the medium into suitable culture vessels under a stream of N2-CO2 (80:20, v/v), seal with butyl rubber stoppers, and autoclave. Prior to inoculation, aseptically and anaerobically add per liter the following solutions (autoclaved and stored under a N2 atmosphere): 

10	ml	Na2S x 9 H2O (5%)	
10	ml	L-Cysteine HCl x H2O (5%)	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water