# J159: THERMOCOCCUS CELER MEDIUM


## Main sol. J159

1000	ml	**Modified Brock's salt base solution**	
40	g	NaCl	
2	g	Yeast extract (BD-Difco)	
10	g	Sulfur (powder)	
0.5	g	Na2S x 9 H2O	
1	mg	Resazurin	

1. Mix ingredients, except yeast extract, sulfur and Na2S x 9H2O, adjust pH to 5.8 with H2SO4, and autoclave under a N2 atmosphere. Separately autoclave yeast extract as a 10% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution, and autoclave under a N2 atmosphere. Aseptically and anaerobically add the yeast extract solution and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and readjust pH to 5.8, if necessary.


## Modified Brock's salt base solution

1.3	g	(NH4)2SO4	
0.28	g	KH2PO4	
0.25	g	MgSO4 x 7 H2O	
0.07	g	CaCl2 x 2 H2O	
2	mg	FeCl3 x 6 H2O	
1.8	mg	MnCl2 x 4 H2O	
4.5	mg	Na2B4O7 x 10 H2O	
0.22	mg	ZnSO4 x 7 H2O	
0.05	mg	CuCl2 x 2 H2O	
0.03	mg	Na2MoO4 x 2 H2O	
0.03	mg	VOSO4 x n H2O	
0.01	mg	CoSO4 x 7 H2O	
1000	ml	Distilled water