# J163: PPLO-GLUCOSE BROTH ## Main sol. J163 21 g PPLO broth (BD-Difco) 5 g Glucose 5 ml Phenol red (0.4%) 200 ml Horse serum (sterile ) 100 ml Baker's yeast (Gibco 360-8180, 25%, fresh extract) 1000000 U Penicillin 700 ml Distilled water 1. Adjust pH to 7.6.