# J18: LACTIC ACID BACTERIA BROTH


## Main sol. J18

10	g	Trypticase peptone (BD-BBL)	
5	g	Yeast autolysate	
12	g	Sodium acetate	
10	g	Glucose	
5	ml	**Solution A**	
5	ml	**Solution A**	
1000	ml	Distilled water	

1. Adjust pH to 5.1-5.3.


## Solution A

10	g	K2HPO4	
10	g	KH2PO4	
100	ml	Distilled water	

1. <I>Solution B: </I> 

4	g	MgSO4 x 7 H2O	
0.2	g	NaCl	
0.2	g	FeSO4 x 7 H2O	
0.2	g	MnSO4 x n H2O	
100	ml	Distilled water