# J19: BIFIDOBACTERIUM MEDIUM


## Main sol. J19

10	g	Casein peptone,  tryptic digest	
5	g	Beef extract (BD-Difco)	
5	g	Yeast extract (BD-Difco)	
10	g	Glucose	
3	g	K2HPO4	
1	ml	Tween 80	
1000	ml	Distilled water	

1. Adjust pH to 6.8.
2. After sterilization, aseptically add solutions of sodium ascorbate and L-cysteine x HCl to final concentration of 1.0% and 0.05%, respectively. Heat medium not freshly prepared in a steamer for 10 min before addition of the reducing substances.