# J194: MMYS-II MEDIUM


## Main sol. J194


## Solution I

1	g	(NH4)2SO4	
8.2	g	NaCl	
0.2	g	MgCl2 x 6 H2O	
0.05	g	CaCl2 x 2 H2O	
1	ml	**Trace element solution SL8**	
3.6	g	Sodium DL-malate	
1	g	Yeast extract	
0.5	g	Na2S2O3 x 5 H2O	
500	ml	Distilled water	


## Solution II

500	ml	Potassium phosphate buffer (pH 7.8, 20 mM)	

1. Separately autoclave Solutions I and II. Mix aseptically and adjust final pH to 7.7-7.8.


## Trace element solution SL8

5.2	g	Na2-EDTA	
1.5	g	FeCl2 x 4 H2O	
70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
62	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
17	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water