# J196: THERMOPROTEUS TENAX MEDIUM


## Main sol. J196

0.264	g	(NH4)2SO4	
0.556	g	FeSO4 x 7 H2O	
0.492	g	MgSO4 x 7 H2O	
0.344	g	CaSO4 x 2 H2O	
0.014	g	KH2PO3	
0.84	mg	NaF	
0.18	mg	MnCl2 x 4 H2O	
0.45	mg	Na2B4O7 x 10 H2O	
22	�g	ZnSO4 x 7 H2O	
5	�g	CuCl2 x 2 H2O	
3	�g	Na2MoO7 x 2 H2O	
1	�g	CoSO4 x 7 H2O	
0.2	g	Yeast extract (BD-Difco)	
5	g	Starch (soluble)	
10	g	Sulfur (powder)	
0.5	g	Na2S x 9 H2O	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Mix ingredients, except yeast extract, starch, sulfur and Na2S x 9H2O, and filter-sterilize while gassing with N2. Autoclave yeast extract and starch as 10% solutions and Na2S x 9H2O as a 5% solution under a N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Aseptically and anaerobically add the yeast extract and starch solutions, and sulfur to the medium. Prior to inoculation, add the sterile Na2S x 9H2O solution, and adjust pH to 5.5 with sterile 1 N H2SO4.