# J21: LACTOBACILLUS MEDIUM III


## Main sol. J21

10	g	Casein peptone,  tryptic digest	
5	g	Yeast extract (BD-Difco)	
2	g	Beef extract (BD-Difco)	
20	g	Glucose	
0.5	g	KH2PO4	
0.5	g	K2HPO4	
10	mg	FeSO4 x 7 H2O	
0.2	g	MgSO4 x 7 H2O	
7.5	mg	MnSO4 x n H2O	
1	ml	Tween 80	
40	ml	Ethanol	
20	g	Sodium acetate	
30	mg	DL-mevalonic acid	
15	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 5.2.