# J229: JCM MEDIUM No. 229


## Main sol. J229

100	g	NaCl	
1	g	KCl	
2	g	MgSO4 x 7 H2O	
10	g	Glucose	
5	g	Tryptone (BD-Difco)	
5	g	Yeast extract (BD-Difco)	
0.5	g	(NH4)2SO4	
1	g	L-Cysteine HCl x H2O	
10	ml	**Trace mineral solution**	
10	ml	**Trace vitamins**	
1	mg	Resazurin	
20	g	Na2CO3	
1000	ml	Distilled water	

1. Check final pH to be about 10.0.


## Trace mineral solution

1.5	g	Nitrilotriacetic acid	
3	g	MgSO4 x 7 H2O	
0.5	g	MnSO4 x n H2O	
1	g	NaCl	
0.1	g	FeSO4 x 7 H2O	
0.1	g	CoCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
0.1	g	ZnSO4 x 7 H2O	
0.01	g	CuSO4 x 5 H2O	
0.01	g	AlK(SO4)2	
0.01	g	H3BO3	
0.01	g	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	

1. Dissolve nitrilotriacetic acid with KOH to pH 6.5, then proceed to add minerals.


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water