# J298: HALOSIMPLEX MEDIUM


## Main sol. J298

2.5	g	Glycerol	
2.5	g	Sodium pyruvate	
0.5	g	K2HPO4	
1	g	(NH4)2SO4	
4	g	KCl	
200	g	NaCl	
20	g	MgSO4 x 7 H2O	
2	ml	**Trace metal solution**	

1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.15.
2. For solid agar slopes or plates, add 20.0 g Bacto agar (BD-Difco).


## Trace metal solution

0.1	g	ZnSO4 x 7 H2O	
0.03	g	MnCl2 x 4 H2O	
0.3	g	H3BO3	
0.2	g	CoCl2 x 6 H2O	
0.01	g	CuSO4 x 5 H2O	
0.02	g	NiCl2 x 6 H2O	
0.03	g	Na2MoO4 x 2 H2O	
100	ml	Distilled water	

1. Adjust pH to 3.0 - 4.0 with HCl.