# J303: PE MEDIUM


## Main sol. J303

5	ml	**Basal salts solution**	
5	ml	**Phosphate solution**	
1	ml	**Vitamin solution**	
0.5	g	(NH4)2SO4	
0.5	g	Na glutamate	
0.5	g	Sodium succinate	
0.5	g	Sodium acetate	
0.5	g	Yeast extract (BD-Difco)	
0.5	g	Casamino acids (BD-Difco)	
0.5	g	Na2S2O3	
1000	ml	Distilled water	

1. Adjust pH to 7.5.


## Basal salts solution

4.12	g	Na3-EDTA	
1.11	g	FeSO4 x H2O	
24.65	g	MgSO4 x 7 H2O	
2.94	g	CaCl2 x 2 H2O	
23.4	g	NaCl	
10	ml	**Trace elements solution**	
1000	ml	Distilled water	


## Phosphate solution

75	g	KH2PO4	
78	g	K2HPO4	
1000	ml	Distilled water	


## Vitamin solution

100	mg	Nicotinic acid	
100	mg	Thiamine HCl	
5	mg	Biotin	
50	mg	p-Aminobenzoic acid	
1	mg	Vitamin B12	
50	mg	Calcium pantothenate	
50	mg	Pyridoxine hydrochloride	
50	mg	Folic acid	
200	mg	Na3-EDTA	
100	ml	Distilled water	


## Trace elements solution

5.58	g	MnSO4 x n H2O	
1.44	g	ZnSO4 x 7 H2O	
1.46	g	Co(NO3)2 x 6 H2O	
1.26	g	CuSO4 x 5 H2O	
1.21	g	Na2MoO4 x 2 H2O	
1.55	g	H3BO3	
20.6	g	Na3-EDTA	
500	ml	Distilled water