# J317: JCM MEDIUM No. 317 ## Main sol. J317 2.2 g Glucose 2.3 g Yeast extract (BD-Difco) 0.14 g KH2PO4 0.2 g MgCl2 x 6 H2O 0.15 g CaCl2 x 2 H2O 0.54 g NH4Cl 2.5 g NaHCO3 0.3 g Na2S x 9 H2O 0.3 g L-Cysteine HCl x H2O 2 ml **Trace vitamins** 1 ml **Trace element solution** 1 ml **Se/W solution** 1 mg Resazurin 1000 ml Distilled water ## Trace element solution 12.8 g Nitrilotriacetic acid 1.35 g FeCl3 x 6 H2O 0.1 g MnCl2 x 4 H2O 0.024 g CoCl2 x 6 H2O 0.1 g CaCl2 x 2 H2O 0.1 g ZnCl2 0.025 g CuCl2 x 2 H2O 0.01 g H3BO3 0.024 g Na2MoO4 x 2 H2O 0.12 g NiCl2 x 6 H2O 1000 ml Distilled water 1. Dissolve nitrilotriacetic acid first in 200 ml of distilled water and adjust pH to 6.5 with KOH. Then add the remaining salts and bring volume to 1.0 L. Readjust pH to 7.0 with KOH. ## Se/W solution 4 mg Na2SeO3 4 mg Na2WO4 x 2 H2O 1000 ml Distilled water ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water