# J333: POTATO-SUCROSE AGAR


## Main sol. J333

200	g	Potato	
20	g	Sucrose	
20	g	Agar	
1000	ml	Distilled water	

1. Adjust pH to 5.6.
2. Wash potatoes with tap water, peel and cut into 1 cm cubes. Rinse with tap water quickly and boil 200 g of potato cubes with 1.0 L of distilled water for 20 min. Mash and squeeze through a muslin bag. Add agar and boil until melting. Add sucrose and mix thoroughly. Make up to 1.0 L, adjust pH and autoclave for sterilization.