# J342: BRAIN HEART INFUSION AGAR WITH SOIL EXTRACT


## Main sol. J342

37	g	Brain heart infusion broth (BD-Difco)	
1000	ml	**Soil extract**	
15	g	Agar	


## Soil extract

1. Suspend 400 g of air-dried garden soil in 1.0 L of tap water and autoclave at 121C for 1 hr. Use the clear supernatant after sedimentation for a few hours and centrifugation.