# J356: MJ BASAL MEDIUM


## Main sol. J356

1000	ml	**MJ(-N) synthetic seawater**	
0.25	g	NH4Cl	
1.5	g	NaHCO3	
1.5	g	Na2S2O3 x 5 H2O	
1	ml	**Trace vitamins**	

1. Mix ingredients, except NaHCO3, Na2S2O3 x 5H2O and Trace vitamins, and autoclave. Filter-sterilize 8% (w/v) NaHCO3, 10% (w/v) Na2S2O3 x 5H2O and Trace vitamins and add to the medium. Replace the gas phase with a N2-CO2-O2 (77:17:6, v/v) gas mixture, and pressurize to 200 kPa.


## MJ(-N) synthetic seawater

30	g	NaCl	
0.14	g	K2HPO4	
0.14	g	CaCl2 x 2 H2O	
3.4	g	MgSO4 x 7 H2O	
4.18	g	MgCl2 x 6 H2O	
0.33	g	KCl	
0.5	mg	NiCl2 x 6 H2O	
0.5	mg	Na2SeO3 x 5 H2O	
0.01	g	Fe(NH4)2(SO4)2 x 6 H2O	
10	ml	**Modified Wolin's mineral solution**	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water