# J358: ATYP MEDIUM


## Main sol. J358

1	ml	**Trace element solution SLA**	
1	ml	**Vitamin solution VA**	
1	g	KH2PO4	
0.5	g	MgCl2 x 6 H2O	
0.1	g	CaCl2 x 2 H2O	
1	g	NH4Cl	
1	g	NaCl	
1	g	Sodium acetate	
1	g	Sodium succinate	
0.5	g	Yeast extract (BD-Difco)	
0.3	g	Bacto peptone (BD-Difco)	
1000	ml	Distilled water	

1. Adjust pH to 6.9.
2. Separately sterilize vitamin solution VA by filtration, and aseptically add to the medium.


## Trace element solution SLA

1800	mg	FeCl2 x 4 H2O	
250	mg	CoCl2 x 6 H2O	
10	mg	NiCl2 x 6 H2O	
10	mg	CuCl2 x 2 H2O	
70	mg	MnCl2 x 4 H2O	
100	mg	ZnCl2	
500	mg	H3BO3	
30	mg	Na2MoO4 x 2 H2O	
10	mg	Na2SeO3 x 5 H2O	
1000	ml	Distilled water	

1. Adjust pH to 2 - 3 with HCl.


## Vitamin solution VA

10	mg	Biotin	
35	mg	Niacin	
30	mg	Thiamine HCl	
20	mg	p-Aminobenzoic acid	
10	mg	Pyridoxine hydrochloride	
10	mg	Calcium pantothenate	
5	mg	Vitamin B12	
100	ml	Distilled water