# J386: MIDDLEBROOK 7H10 AGAR WITH OADC ENRICHMENT


## Main sol. J386

19	g	Middlebrook 7H10 agar (BD-Difco)	
5	ml	Glycerol	
895	ml	Distilled water	

1. Sterilize at 121C for 10 min and cool to 50 - 55C. Aseptically add 100 ml of Middlebrook OADC Enrichment (BD-BBL) and mix thoroughly.