# J399: DESULFOVIBRIO MAGNETICUS MEDIUM ## Main sol. J399 0.2 g K2HPO4 0.06 g NH4Cl 2 ml **Fe(III) quinate solution** 4 ml **Trace minerals** 8 ml **Trace vitamins** 0.58 g Sodium fumarate 0.44 g Sodium pyruvate 0.05 g L-Cysteine HCl x H2O 1000 ml Distilled water 1. Adjust pH to 7.0. Prepare medium anaerobically under a N2 gas atmosphere. ## Fe(III) quinate solution 0.45 g FeCl3 x 6 H2O 0.19 g Quinic acid 100 ml Distilled water ## Trace minerals 1.5 g Nitrilotriacetic acid 3 g MgSO4 x 7 H2O 0.5 g MnSO4 x n H2O 1 g NaCl 0.1 g FeSO4 x 7 H2O 0.1 g CoSO4 x 7 H2O 0.1 g CaCl2 x 2 H2O 0.1 g ZnSO4 x 7 H2O 0.01 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 1000 ml Distilled water 1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water