# J401: PROPIONIVIBRIO MEDIUM ## Main sol. J401 0.2 g KH2PO4 0.25 g NH4Cl 1 g NaCl 0.4 g MgCl2 x 6 H2O 0.5 g KCl 0.15 g CaCl2 x 2 H2O 1 ml **Trace minerals** 10 ml **Trace vitamins** 1 ml Quinic acid (5 mM) 1 mg Resazurin 2.5 g NaHCO3 0.9 g 2,3-butanediol 0.36 g Na2S x 9 H2O 1 ml Distilled water 1. Adjust pH to 7.2. Prepare medium anaerobically under a N2-CO2 (4:1, v/v) gas mixture. ## Trace minerals 1.5 g Nitrilotriacetic acid 3 g MgSO4 x 7 H2O 0.5 g MnSO4 x n H2O 1 g NaCl 0.1 g FeSO4 x 7 H2O 0.1 g CoSO4 x 7 H2O 0.1 g CaCl2 x 2 H2O 0.1 g ZnSO4 x 7 H2O 0.01 g CuSO4 x 5 H2O 0.01 g AlK(SO4)2 0.01 g H3BO3 0.01 g Na2MoO4 x 2 H2O 1000 ml Distilled water 1. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0. ## Trace vitamins 2 mg Biotin 2 mg Folic acid 10 mg Pyridoxine hydrochloride 5 mg Thiamine HCl 5 mg Riboflavin 5 mg Nicotinic acid 5 mg Calcium pantothenate 0.1 mg Vitamin B12 5 mg p-Aminobenzoic acid 5 mg Lipoic acid 1000 ml Distilled water