# J402: HALOCOCCUS DOMBOWSKII MEDIUM


## Main sol. J402

5	g	Casamino acids (BD-Difco)	
5	g	Yeast extract	
12.1	g	Tris base	
2	g	KCl	
20	g	MgCl2 x 6 H2O	
0.2	g	CaCl2 x 2 H2O	
200	g	NaCl	

1. Add components to distilled water and bring volume to 1.0 L.
2. Adjust pH to 7.4.
3. For preparation of solid medium, add 20.0 g/L agar.
4. Comment: For cultivation of JCM 19729, supplement with 10.0 g/L sea salts (Sigma).