# J430: DESULFOSPOROMUSA MEDIUM


## Main sol. J430

3	g	Na2SO4	
0.2	g	KH2PO4	
0.3	g	NH4Cl	
1	g	NaCl	
0.4	g	MgCl2 x 6 H2O	
0.5	g	KCl	
0.15	g	CaCl2 x 2 H2O	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
1	mg	Resazurin	
900	ml	Distilled water	

1. Mix components thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas atmosphere. After cooling, aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized): 

20	ml	Sodium lactate (*, 1.0 M)	
10	ml	Yeast extract (10%)	
10	ml	**Trace vitamins** (*)	
60	ml	NaHCO3 (*, 8%)	
8	ml	Na2S x 9 H2O (5%)	

2. Adjust pH to 7.1 - 7.4, if necessary.


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water	


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water