# J483: ETHANOLOGENBACTERIUM MEDIUM


## Main sol. J483

10	g	Glucose	
4	g	Tryptone (BD-Difco)	
2	g	Beef extract (BD-Difco)	
1	g	Yeast extract (BD-Difco)	
4	g	NaCl	
1.5	g	K2HPO4	
0.1	g	FeSO4 x 7 H2O	
0.2	g	MgCl2 x 6 H2O	
0.5	g	L-Cysteine HCl x H2O	
10	ml	**Microelement solution**	
10	ml	**Vitamin solution**	
1000	ml	Distilled water	

1. Mix components except vitamin solution, and adjust pH to 4.5 - 6.5. After autoclaving, aseptically add filter-sterilized vitamin solution.


## Microelement solution

0.01	g	MnSO4 x n H2O	
0.05	g	ZnSO4 x 7 H2O	
0.01	g	H3BO3	
4.5	g	Nitrilotriacetic acid	
0.01	g	CaCl2 x 2 H2O	
0.01	g	Na2MoO4 x 2 H2O	
0.2	g	CoCl2 x 6 H2O	
0.01	g	AlK(SO4)2 x 12 H2O	
1000	ml	Distilled water	


## Vitamin solution

0.01	g	Cyanocobalamin	
0.025	g	Ascorbic acid	
0.025	g	Riboflavin	
0.02	g	Citric acid	
0.05	g	Pyridoxine hydrochloride	
0.01	g	Folic acid	
0.01	g	p-Aminobenzoic acid	
0.025	g	Creatine	
1000	ml	Distilled water