# J545: DESULFOTOMACULUM Lam5 MEDIUM


## Main sol. J545

0.3	g	KH2PO4	
0.3	g	K2HPO4	
1	g	NH4Cl	
1	g	NaCl	
3	g	Na2SO4	
0.1	g	KCl	
0.1	g	CaCl2	
0.5	g	MgCl2 x 6 H2O	
0.1	g	Yeast extract	
1	g	Sodium lactate	
10	ml	**Trace element solution**	
2	g	NaHCO3	
0.5	g	L-Cysteine HCl x H2O	
0.4	g	Na2S x 9 H2O	
1	mg	Resazurin	
1000	ml	Distilled water	

1. Mix ingredients except NaHCO3, L-cysteine x HCl x H2O and Na2S x 9H2O and adjust pH to 7.2. Distribute the medium into tubes (e.g., 5 ml of the medium in the Hungate tubes) under a N2 x CO2 gas mixture (4:1, v/v), seal with butyl rubber stoppers and autoclave. After cooling, add solutions of NaHCO3 [5%, filter-sterilized and stored under a N2-CO2 (4:1, v/v) gas mixture], L-cysteine x HCl x H2O and Na2S x 9H2O (5%, autoclaved and stored under N2) to the medium.


## Trace element solution

12.5	ml	HCl (25%)	
2.1	g	FeSO4 x 7 H2O	
30	mg	H3BO3	
100	mg	MnCl2 x 4 H2O	
190	mg	CoCl2 x 6 H2O	
24	mg	NiCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
144	mg	ZnSO4 x 7 H2O	
36	mg	Na2MoO4 x 2 H2O	
987	ml	Distilled water