# J592: MODIFIED MMJS MEDIUM (B)


## Main sol. J592


## Solution A

20	g	NaCl	
0.09	g	K2HPO4	
0.07	g	KH2PO4	
0.8	g	CaCl2 x 2 H2O	
1	g	NH4Cl	
1	g	NaNO3	
1	g	MgSO4 x 7 H2O	
6	g	MgCl2 x 6 H2O	
0.33	g	KCl	
1	mg	NiCl2 x 6 H2O	
1	mg	Na2SeO3 x 5 H2O	
0.01	g	Fe2(SO4)3 x n H2O	
1	mg	H2WO4	
3	g	Na2S2O3 x 5 H2O	
0.01	g	FeSO4 x 7 H2O	
0.01	g	Ferrous citrate	
5	ml	**Trace mineral solution**	
1000	ml	Distilled water	

1. Mix ingredients and adjust pH to 6.8 with NaOH. Distribute the solution into culture vessels (leaving more than 80% volume of gas phase in the vessel) under a stream of a N2-CO2 (80:20, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. Then, add the following solutions (filter-sterilized) to the medium (per liter): 

1	ml	**Trace vitamins**	
20	ml	NaHCO3 (5%)	

2. Finally, replace the gas phase with a N2-CO2-O2 (79:20:1, by volume) gas mixture and pressurize to 200 kPa with the same gas mixture.


## Trace mineral solution

1. Use Trace mineral solution of Medium No. 413 with 0.01 g/L (final) Na2MoO4 x 2H2O.


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water