# J593: MODIFIED 9K MEDIUM FOR FERROPLASMA AEOLICUM


## Main sol. J593

0.4	g	MgSO4 x 7 H2O	
0.2	g	(NH4)2SO4	
0.1	g	KCl	
0.1	g	K2HPO4	
25	g	FeSO4 x 7 H2O	
0.2	g	Yeast extract	
1	ml	**FeCl2 solution**	
1	ml	**Trace element solution**	
1000	ml	Distilled water	

1. Adjust pH to 1.6 - 1.7 with 10 N H2SO4 and autoclave. After cooling, add filter-sterilized K2S4O6 solution to give a final concentration of 5 mM.


## FeCl2 solution

10	ml	HCl (7.7 M, 25%)	
1.5	g	FeCl2 x 4 H2O	
990	ml	Distilled water	


## Trace element solution

70	mg	ZnCl2	
100	mg	MnCl2 x 4 H2O	
6	mg	H3BO3	
190	mg	CoCl2 x 6 H2O	
2	mg	CuCl2 x 2 H2O	
24	mg	NiCl2 x 6 H2O	
36	mg	Na2MoO4 x 2 H2O	
1000	ml	Distilled water