# J599: MODERATE HALOPHILE MEDIUM


## Main sol. J599

10	g	Yeast extract	
5	g	Proteose peptone no. 3 (BD-Difco)	
1	g	Glucose	
81	g	NaCl	
9.6	g	MgSO4 x 7 H2O	
7	g	MgCl2 x 6 H2O	
0.36	g	CaCl2 x 2 H2O	
2	g	KCl	
0.06	g	NaHCO3	
0.026	g	NaBr	

1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2. For preparation of solid medium, add 20.0 g/L agar.