# J601: ANCALOMICROBIUM MEDIUM


## Main sol. J601

0.25	g	(NH4)2SO4	
0.25	g	Glucose	
10	ml	**Vitamin solution**	
20	ml	**Modified Hutner's basal salts**	
0.071	g	Na2HPO4	
980	ml	Distilled water	

1. Adjust pH to 7.0.
2. Separately sterilize Vitamin solution by filtration and aseptically add to the medium.


## Vitamin solution

0.1	mg	Vitamin B12	
2	mg	Biotin	
5	mg	Thiamine-HCl x 2 H2O	
5	mg	Calcium pantothenate	
2	mg	Folic acid	
5	mg	Riboflavin	
5	mg	Nicotinamide	
1000	ml	Distilled water	

1. Filter-sterilize.


## Modified Hutner's basal salts

10	g	Nitrilotriacetic acid (NTA)	
29.7	g	MgSO4 x 7 H2O	
3.335	g	CaCl2 x 2 H2O	
9.25	mg	(NH4)6Mo7O24 x 4 H2O	
99	mg	FeSO4 x 7 H2O	
50	ml	**Metals ''44''**	
950	ml	Distilled water	

1. Dissolve nitrilotriacetic acid and adjust pH to 7.0 with KOH (about 7.3 g). Then proceed to add salts. Readjust pH to 6.8 with NaOH or H2SO4.


## Metals ''44''

250	mg	Na2-EDTA	
1095	mg	ZnSO4 x 7 H2O	
500	mg	FeSO4 x 7 H2O	
154	mg	MnSO4 x n H2O	
39.2	mg	CuSO4 x 5 H2O	
24.8	mg	Co(NO3)2 x 6 H2O	
17.7	mg	Na2B4O7 x 10 H2O	
1000	ml	Distilled water