# J642: MOORELLA AN10 MEDIUM


## Main sol. J642


## Solution A

1	g	Yeast extract	
1	g	Casitone (BD-Difco)	
0.348	g	K2HPO4	
0.227	g	KH2PO4	
0.5	g	NH4Cl	
0.5	g	MgSO4 x 7 H2O	
0.25	g	CaCl2 x 2 H2O	
2.25	g	NaCl	
0.1	g	FeSO4 x 6 H2O	
10	ml	**Trace vitamins**	
1	ml	**Trace element solution**	
1	ml	**Se/W solution**	
0.5	g	L-Cysteine HCl x H2O	
0.5	g	Na2S x 9 H2O	
1	mg	Resazurin	
850	ml	Distilled water	

1. Mix components and autoclave under a N2-CO2 (4:1, v/v) atmosphere.


## Solution B

5	g	Fructose	
100	ml	Distilled water	

1. Autoclave under a N2 atmosphere.


## Solution C

20	ml	NaHCO3 (8%)	

1. Filter-sterilize and store under a N2-CO2 (4:1, v/v) atmosphere.
2. To complete the medium, mix Solutions A, B and C under a N2-CO2 (4:1, v/v) atmosphere. Reduce the medium with 0.3 g/L L-cysteine x HCl x H2O and 0.3 g/L Na2S x 9H2O from sterile stock solutions (autoclaved and stored under a N2 atmosphere).


## Trace vitamins

2	mg	Biotin	
2	mg	Folic acid	
10	mg	Pyridoxine hydrochloride	
5	mg	Thiamine HCl	
5	mg	Riboflavin	
5	mg	Nicotinic acid	
5	mg	Calcium pantothenate	
0.1	mg	Vitamin B12	
5	mg	p-Aminobenzoic acid	
5	mg	Lipoic acid	
1000	ml	Distilled water	


## Trace element solution

1. Use Trace element solution of Medium No. 243 without Na2SeO3 x 5H2O.


## Se/W solution

4	mg	Na2SeO3	
4	mg	Na2WO4 x 2 H2O	
1000	ml	Distilled water