# J652: TRYPTICASE SOY AGAR WITH 0.5 x ARTIFICIAL SEAWATER


## Main sol. J652

40	g	Trypticase soy agar (BD-BBL)	
1000	ml	**0.5 x Artificial seawater**	

1. Adjust pH to 7.0.


## 0.5 x Artificial seawater

15	g	NaCl	
0.35	g	KCl	
5.4	g	MgCl2 x 6 H2O	
2.7	g	MgSO4 x 7 H2O	
0.5	g	CaCl2 x 2 H2O	
1000	ml	Distilled water